In vitro Rodent Models of Parkinson’s Disease
- Mouse primary striatal neurons are the most sensitive neurons to the oligomers of mouse or human α-synuclein (αSO), more than cortical, cerebellar, or hippocampal neurons.
- 400 nM αSO kill ̴ 40% of mouse primary striatal neurons within 72 h.
- Typical in vitro assay results for antibodies or small molecules are shown below (Figure 1)
- Various fibrillar forms of α-synuclein, made by multiple academic labs, show similar dose-dependent neurotoxicity on primary striatal neurons after 72 h. However, SynAging’s αSO are the most toxic conformation (Figure 2)
- BDNF (50 nM) is a suitable positive control preventing αSO neurotoxicity (Figure 3).
- SynAging's high reproducibility for producing misfolded α-synuclein aggregates with the same effects is shown at the end of the page.
Product Sheet “In Vitro Neuroprotection Screen for Parkinson’s Disease” Download PDF, 890 KB
- primary mouse striatal neurons are seeded in 48-well plates
- at day 11, medium is supplemented with 400nM αSO preparation
- neuronal viability is determined 72h later with the MTT or ATP-Light assay
- test items can be added before, together with, or after the challenge with αSO
- standard plate layout is in triplicates, including vehicle control, negative control (αSO), positive control (αSO and BDNF), and compounds controls (no αSO)
- In one 48 well plate (design below):
6 compounds can be tested at a single concentration,
2 compounds can be tested at three concentrations,
or one compounds in dose response mode, six concentrations
- αSO-treated neurons show (Figure 4):
- severe cell shrinkage
- destruction of the neuronal network
- phenotype different from AβO
- slower damage than AβO
- αSO preparation is highly reproducible in the resulting neurodegenerative properties (Figure 5)
Product Sheet “In Vitro Neuroprotection Screen for Parkinson’s Disease”, Download PDF, 2.2 MB